DLAR USER MANUAL FOR RESEARCHERS
- Basic Room听 Information
- Cage Docking
- Weaning
- Chemical Hazards
- Clinical Concerns
- Post Procedure/Chronic Care Monitoring
- Open Incision Guideline
- Animal Bite
- Needlestick
- 听
Basic Room information
Light Cycle听
Temperature
Relative Humidity
Cage Change
Census Count
Room Cleaning
Hood Cleaning
Tech Time Fee
Senior Tech Time Fee
~7:00 AM - 7:00 PM DST (~March - November)
~6:00 AM - 6:00 PM EST (~November - March)
Adjustments require IACUC/DLAR approval.
词70-74掳贵
Adjustments require IACUC/DLAR approval.
REPORT ABNORMALITIES TO DLAR!
~30-70%
REPORT ABNORMALITIES TO DLAR!
~Every 2 weeks (or as needed)
Adjustments require IACUC/DLAR approval.
~Weekly (or as needed)
~Weekly (or as needed)
~Monthly (or as needed)
$30/hour - charged in 15 minute increments
(weaning, euthanasia, separation of cages, maloccluson TX's, etc.)
$45/hour - charged in 15 minute increments
(injections, genotyping, surgical-post op monitoring, etc.)
Cage Docking
Placing cages on the rack correctly ensures the cage has complete access to water and HEPA filtered air.
Look for the Colored Dot!
If you see the RED DOT, you cage is NOT on the rack correctly! Remove your cage and try again.
Weaning
Mouse
1. Supply wet food and/or Boost in petri dish.
听 听 听- One Boost can supply up to 3 cages.
2. Place a few pieces of animals' food on the cage floor.
3. Put the "Check Lixit" slip on the cage and make sure lixit works.
Check Lixit Slips
EXPECTING CARD =听
CHECK LIXIT SLIPS
Rat/Vole
1. Supply wet food in crock or paper cup.
2. Place a few pieces of animals' food on the cage floor.
3. Put the "Check Lixit" slip on the cage and make sure lixit works.
Check Lixit Slips
EXPECTING CARD =听
CHECK LIXIT SLIPS
听
Chemical Hazards
1. Attend DLAR Hazad training first.
2. Be sure to send you hazard door sign to DLAR at least 5 days before starting.
3. Be sure to wear all personal protective equipment (PPE).
-
- Blue chemical gown may be required. See door sign.
4. Always handle animals and cages in the hood.
-
- Must wear N95 mask* if working outside of the hood.
*Requires fit testing with Environmental Health and Radiation Safety (419.383.5089)
- Must wear N95 mask* if working outside of the hood.
5. Use chemical hazard labels and fill out completely.
6. If a label is crossed off:
-
- Empty cages can be taken to cage wash
- Use regular carcass bags.
7. If label is听NOT crossed off:
-
- DO NOT take empty cages to cage wash.
- Use red hazard carcass bag.
If you have any questions, see a DLAR staff member.
Clinical Concerns
*Report Emergencies to the Veterinary Staff Immediately*
- Using a Veterinary Care Card (VCC), document the animals' condition. Fill out as muc informaiton as possible.
- The VCC is 2 parts. remove the top part and turn it in to the Vet Tech office.
听 听 听- Leave the 2nd part (carbon copy) on the cage. - The clinical team will evaluate the animal(s) as soon as possible and will contact you with updates.
- If you find a VCC on a cage that you are euthanizing, complete the euthanasia section (yellow) and turn it in to the Vet Tech office.
Important Contacts
- Dr. Lisa Root
419.383.4314
- Jamie Harris
419.383.1063
- Kimberly O'Meara-Schmidt
419.383.1499
Post Procedure/Chronic Care Monitoring
- The yellow Post Procedural/Chronic Care card and it's supporting document, the Monitoring and Supportive Care Log, must be used whenever a procedure, surgery, or phenotypic abnormality occurs which will impact the animal's health over time (i.e.: tumor growth, arthritis, paralysis, sepsis, aging, runted pups, etc.).
- The Post Procedural/Chronic Care card听must be placed in front of the origical cage card as soon as the adverse health effects are visible. This card will stay on the cage for the life of the animal and then may be kept for your records after the animal is euthanized.
- The Monitoring and Supportive Care Log, located in the yellow clinical binder in the animal room, must also be completed, if not providing your own monitoring log. Be sure to include all monitoring and treatments and their frequency.
Open Incision Guideline
What do I do if the skin sutures or wound clips fall out and the incision is open?
- MUST consult with DLAR upon first observation of missing wound clips/sutures and/or
an open incision.
- Record the observation on the back of the green surgery card.
- Record the observation on the back of the green surgery card.
- DLAR will assess the animal(s) to determine the best treatment plan. Here are possible
treatment outcomes.
- Grade 0
鈥⑻� 1 skin wound clip/suture is missing but the skin incision is still closed/sealed
鈥⑻� No treatment (continue to monitor) - Grade 1
鈥⑻� 1-2 skin wound clips/sutures are missing causing a small (<3mm) gap in the skin with a little pink or white tissue
听 听 visible
鈥⑻� Treatment: Neosporin plus pain relief (a.k.a. TAO+) daily for 3 days - Grade 2
鈥⑻� >2听skin wound clips/sutures are missing causing a medium (3-5mm) gap in the skin with a some pink or white
听 听 tissue visible
鈥⑻� Treatment: Gently clean the wound, Neosporin plus pain relief (a.k.a. TAO+) daily for 7 days, and more frequent
听 听 cage changing. - Grade 3
鈥⑻� >2听skin wound clips/sutures are missing causing a large (>5mm) gap in the skin with a lot of tissue visible
鈥⑻� Treatment depends on how much time has elapsed since the surgery and the condition of the tissue. Call the
听 听 Veterinarian (or Vet Techs) during business hours) for further instructions. - Grade 4
鈥⑻�Skin and body wall incisions are open, and organs are protruding out of the body cavity
鈥⑻� EUTHANIZE IMMEDIATELY!
- Grade 0
- In all the above cases, the veterinarian or the DLAR staff must be consulted so we
can discuss the situation and determine the best course of action for the animal's
health and welfare.
- After hours, on weekends or holidays, you will need to initiate treatment based on
the above criteria, and you will need to notify DLAR.
- Document the treatment on the back of the green surgery card.
- Phone numbers for Dr. Root and Ashley Kurth are posted in the facility.
- Dr. Root and Ashley Kurth also check emails throughout the weekend.
Got bit? - What to do in case of an animal bite
Clean It
- Thoroughly wash the wound with warm water and disinfect with soap.
- Cover it with a waterproof band-aid/dressing.
Report It
- Report the incident to your supervisor immediately and notify DLAR management.
- An Injury/Illness Report Form must be completed for any incident resulting in an injury or illness to an employee or student employee at 兔子先生传媒文化作品.
Treat It
- Discuss with your physician, EH&RS/Occupational Health (for referral to UTMC Emergency Department or Family Medicine), and/or supervisor about seeking further medical treatment.
For any questions, please contact Enironmental Health & Radiation Safety at 419.383.5089.
Got Stuck? - What to do in case of a needlestick
Clean It
- Thoroughly wash the wound with warm water and disinfect with soap.
- Cover it with a waterproof band-aid/dressing.
Report It
- Report the incident to your supervisor immediately and notify DLAR management.
- An Injury/Illness Report Form must be completed for any incident resulting in an injury or illness to an employee or student employee at 兔子先生传媒文化作品.
Treat It
- Exposed individual will go to the UTMC Emergency Department.
- UTMC Emergency Department will provide the exposed individual with PERRP Sharps Injury Form Needlestick Report. This must be completed in addition to the Injury/Illness Report Form.